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PS 3217-2-2026
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Soil quality — Determination of soil microbial biomass — Part 2: Fumigation-extraction method
Committee:
Environment
Sector:
Environment
Available Standards
Original Standard
إنجليزي (English)
18
Page
2026
International Classification for Standards (ICS)
| Code | Classification |
|---|---|
| 13.080.30 | Biological properties of soils |
Adopted Standards
| Standards / Amendment / Corrigendum | |
|---|---|
| 1 | ISO 14240-2:1997 |
Replacement Sequence
| Replaces |
|---|
| PS 3217-2-2015 |
Scope
This part of PS 3217 specifies a method for the estimation of microbial biomass of soils by measurement of total extractable organic biomass material mainly from freshly killed microorganisms. The method is also applicable to the estimation of microbial nitrogen and microbial ninhydrin-reactive nitrogen in soil, but this part of PS 3217 describes only the measurement of extractable organic carbon. The fumigation-extraction (FE) method is applicable to aerobic and anaerobic (water-logged, paddy) soils over the whole range of soil pH. Biomass can be also measured in soils containing actively decomposing substrates and soils supersaturated with potassium sulfate solution.
NOTE — Chloroform fumigation also affects soil fauna. The contribution of carbon from these organisms is generally small (< 5 %) and can usually be neglected.